Description : Cl. Difficile has been demonstrated as a major cause of antibiotic associated diarrhea and Pseudo membranous colitis in man. This medium is designed for the selective isolation and enumeration of CI. Difficile from faecal specimens. The selective agents employed Cefoxitin and D – Cycloserine inhibit the growth of most Enterrobacteriaceae, Streptococci, Bacteroides and other Clostridial species.