Letheen Broth Modified is used for the microbiological testing of cosmetics. Beef extract, included in the Letheen Broth bases, and peptone provide carbon and nitrogen sources required for good growth of a wide variety of bacteria and fungi. The peptone level was increased in the modified Letheen Broth formula to provide for better growth. Vitamins and cofactors, required for growth as well as additional sources of nitrogen and carbon, are provided by yeast extract. Sodium chloride provides a suitable osmotic environment. This media also contain polysorbate 80, lecithin and sodium bisulphite to partially neutralize the preservative systems commonly found in cosmetics.

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