Yeast and mould agar is recommended for the isolation and maintenance of yeast and moulds. Detection and enumeration of yeasts in the presence of moulds may be made easier by using a combined anaerobic/aerobic incubation procedure. Cultures are initially incubated at 25C for 3 days under anaerobic conditions and then for a further two days aerobically. Development of mould colonies is impeded during the anaerobic phase of incubation. Yeast colonies may be very small immediately following anaerobic incubation but will increase in size in air. Mould growth may become completely unrestricted after 3 days in air. Dimorphic moulds may form yeast- like colonies during anaerobic incubation.

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